Fig. 5

Muscle-derived stem cell (MDSC) engraftment in pancreatic islets from streptozotocin (STZ)-treated mice 48 h after intraperitoneal injection (IP). a PP8 MDSC labeled with the cell tracker membrane dye DiI were administered by IP injection into mice 1 h after IP injection of either saline solution (control) or streptozotocin (STZ treated), and the pancreas was removed for cryosection analysis 48 h later. b Shown are representative fluorescence micrographs of sections from untreated control (upper panels) and STZ-treated mice (bottom panels). Left panels, DiI fluorescence (red); right panels, Hoechst staining for DNA (blue). Scale bar = 100 μm. c Islet engraftment of DiI-labeled cells in STZ-treated and untreated mice in comparison to total cell nuclei counted per islet in the pancreatic cryosections analyzed. Represented in the histogram are the average number of cells counted per islet section and DiI MDSC engrafted in control and STZ-treated islets. *P < 0.01, **P < 0.001