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Table 2 Dose-dependent effects of MSC treatment on leucocyte infiltration, nerve fibre density and number of myenteric neurons in the inflamed distal colon

From: The neuroprotective effects of human bone marrow mesenchymal stem cells are dose-dependent in TNBS colitis

  Sham TNBS TNBS + 1 × 105 MSCs TNBS + 1 × 106 MSCs TNBS + 3 × 106 MSCs
Total no. CD45-IR cells
 Mucosa 341 ± 34 927 ± 31### 686 ± 23***### 368 ± 27***^^^ 425 ± 25***^^^
 Muscle 33 ± 4 131 ± 1### 83 ± 5***### 32 ± 6***^^^ 29 ± 6***^^^
β-tubulin (III)-IR fibre density
 Mucosa 3.31 ± 0.09 1.68 ± 0.05### 2.54 ± 0.05***### 3.39 ± 0.08***^^^ 3.35 ± 0.04***^^^
 Muscle 5.54 ± 0.17 4.03 ± 0.11### 3.93 ± 0.07### 5.67 ± 0.10***^^^ 5.50 ± 0.12***^^^
Total no. of Hu-IR myenteric neurons
 Ganglia 124 ± 2 108 ± 5# 104 ± 2## 128 ± 3**^^ 127 ± 4**^^
 Area 1498 ± 13 1330 ± 41## 1312 ± 20### 1510 ± 19***^^ 1513 ± 12***^^^
Total no. of nNOS-IR myenteric neurons
 Ganglia 22 ± 1 27 ± 1## 27 ± 1### 20 ± 1***^^ 22 ± 1**^^^
 Area 262 ± 12 318 ± 7### 317 ± 5### 277 ± 6**^^ 246 ± 3***^^^
Proportion of nNOS-IR myenteric neurons
 Ganglia 17 ± 1 26 ± 2### 27 ± 1### 16 ± 1***^^^ 18 ± 1***^^^
 Area 17 ± 1 24 ± 1### 24 ± 1### 18 ± 1***^^^ 16 ± 1***^^^
Total no. of ChAT-IR myenteric neurons
 Ganglia 67 ± 1 52 ± 2## 57 ± 2## 64 ± 2**^ 65 ± 3**^
 Area 749 ± 29 545 ± 24### 585 ± 23## 708 ± 29**^ 743 ± 14***^^
Proportion of ChAT-IR myenteric neurons
 Ganglia 54 ± 1 49 ± 1 55 ± 1 50 ± 1 52 ± 4
 Area 50 ± 2 41 ± 1## 45 ± 2 47 ± 2* 49 ± 1*
  1. MSC mesenchymal stem cell, TNBS 2,4,6-trinitrobenzene sulfonic acid, IR immunoreactive, nNOS neuronal nitric oxide synthase, ChAT Choline acetyltransferase
  2. # P < 0.05, ## P < 0.01, ### P < 0.001 when compared to sham-treated guinea-pigs
  3. *P < 0.05, **P < 0.01, ***P < 0.001 when compared to TNBS-only administered guinea-pigs
  4. ^P < 0.05, ^^P < 0.01, ^^^P < 0.001 when compared to 1 × 105 MSC-treated guinea-pigs
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Stem Cell Research & Therapy

ISSN: 1757-6512