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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Autophagy mediates the beneficial effect of hypoxic preconditioning on bone marrow mesenchymal stem cells for the therapy of myocardial infarction

Fig. 3

Effect of hypoxic preconditioning (HPC) on the apoptosis of BM-MSCs. a Representative immunofluorescence images of terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) (green fluorescence) and 4,6-diamidino-2-phenylindole (DAPI) (blue fluorescence) in BM-MSCs under normal conditions and after hypoxia/serum deprivation (H/SD) (Scale bars = 20 μm). b Quantification of the apoptotic BM-MSCs was presented as the percentage of apoptotic cells. c Histogram illustrated the caspase-3 enzymatic activity in BM-MSCs in all groups. Data are expressed as means ± SEM; n = 5; *p < 0.05H/SD vs. Normal, # p<0.05

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