Fig. 5
Autophagy modulated the protective effect of hypoxic preconditioning (HPC) on BM-MSCs. a Representative terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) images of BM-MSCs treated by HPC for 24 h with or without autophagy inhibition by 3-methyladenine (3-MA) and Atg7 small interfering RNA (siRNA) (Scale bars = 20 μm). b Representative in vitro BLI results of BM-MSCsFluc+GFP+ in all groups. c The quantification of the apoptotic BM-MSCs. d The quantification of the in vitro BLI assays. Data are expressed as means ± SEM; n = 5; *p < 0.05. DAPI 4,6-diamidino-2-phenylindole, H/SD hypoxia/serum deprivation