Fig. 6
Evaluation of the survival of transplanted BM-MSCFluc+GFP+. a Representative longitudinal BLI spatiotemporally tracked BM-MSC Fluc+GFP+ survival in normal preconditioned mesenchymal stem cells (NPC MSCs) (top row, n = 10), hypoxic preconditioned MSCs (HPC MSCs) (second row, n = 10), the HPCMSCs + 3-methyladenine (3-MA) group (third row, n = 10), and the HPCMSCs + Atg7 small interfering RNA (siRNA) group (bottom row, n = 10). Color scale bar values are in photons/s/cm2/sr. b Quantitative analysis of Firefly luciferase (Fluc) optical signals on fixed regions of interest (ROI). c Representative confocal laser microscopic images of engrafted BM-MSCFluc+GFP+ (green fluorescent protein (GFP): green fluorescence), cTnI (red, upper panel), terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) (red, middle panel), Ki67 (red, lower panel), and 4,6-diamidino-2-phenylindole (DAPI) (blue fluorescence) at 2 weeks after transplantation. Scale bar = 50 μm. Quantitative analysis of the ratio of GFP/DAPI (d), GFP and TUNEL double-positive cells (GFP+TUNEL+/DAPI, e) and GFP and Ki67 double-positive cells (GFP+Ki67+/DAPI, f) in all groups. Data are expressed as means ± SEM; n = 5; *p < 0.05