Fig. 2

IF analysis of the distribution of LRCs and co-expression of cell lineage markers in transversal sections of postnatal day 5 (P5) mouse prostates. Paraffin sections were subjected to antibodies against BrdU (a–e; green), p63 (a–c), AR (d, e), and negative control (f). Note that both basally (p63-positive; arrowheads) and luminally (p63-negative; arrows) located LRCs were more numerous and more strongly labeled in ducts (a,b) than when compared to distal epithelium (a, c). In P5 prostates, AR was expressed in terminal ducts (d), and some of the strongest AR-expressing cells co-expressed BrdU (arrow, d). However, distal acini (e) expressed no or only low levels of AR (arrowheads indicate stromal AR expression in d, e). DAPI was used as counterstaining in a–e. Scale bars = 200 μm (a), 100 μm (f), and 50 μm (b–e)