Fig. 4
Residual infarct size 9 days post MCAo and cell migration analysis. a Differences in residual infarct volumes obtained 9 days post MCAo are shown for all treatment groups. Groups are controls (n = 9; n = 5 for MCAo = 45 min; n = 4 for MCAo = 90 min), and animals treated with 5 × 104 ASCs (n = 7; all MCAo = 45 min), 3 × 105 ASCs (n = 7; n = 6 for MCAo 45 min; n = 1 for MCAo = 90 min), and 1 × 106 ASCs (n = 5; n = 3 for MCAo = 45 min; n = 2 for MCAo = 90 min). The median for each group is indicated as a red dotted line. Each column represents residual infarct size for one animal assessed as a percentage of the individual infarct size obtained 48 h post MCAo for each individual animal. *p < 0.05 for 3 × 105 ASC group vs. control. b Migration activity in the ischaemic hemisphere (image 1, dotted line) of one animal treated with 3 × 105 ASCs is shown. A strong hypointense signal in the right ventricle of the appending T2*-weighted image (image 2) obtained after 48 h decreases within 9 days post MCAo with a new hypointense spot appearing in the striatum close to the infarct area (image 3). Signals are confirmed by voxel analysis. Histological analysis of the same location reveals positive PB staining (blue signals, some supported by arrows in image 4, location # and §). ASC adipose tissue - derived stem cell, MCAo middle cerebral artery occlusion, PB Prussian blue