Fig. 5
Effect of PGE2 on cytokine levels produced by monocytes from septic patients. Monocytes from septic patients (1 × 106 PBMC/well in six-well plates) and ASCs (250,000 cells/well) were cultured alone or cocultured directly for 24 h. In another group, monocytes were incubated with exogenous PGE2 (4 ng/ml) for 24 h. Separately, monocyte-ASC cocultures were treated with NS-398 (1 μM) for 24 h. TNF-α (a) and IL-10 (b) levels in the culture supernatant were determined via ELISA. Data are expressed as mean ± SEM; n = 7 per group. *p < 0.05. ASC adipose-derived mesenchymal stem (stromal) cell, IL interleukin, MO monocytes, PGE2 prostaglandin E2, TNF tumor necrosis factor