Fig. 6

Effect of ASCs on F4/80⁺CD11b⁺Ly6C^high monocyte population in vivo. Representative flow cytometry plots of the F4/80⁺CD11b⁺Ly6C^high and F4/80⁺CD11b⁺Ly6C^low populations from the mouse peripheral blood are shown in the top panel. Monocytes were initially gated by SSC vs FSC (a) followed by F4/80 and CD11b (b). The F4/80⁺CD11b⁺ cell populations were further gated with Ly6C for F4/80⁺CD11b⁺Ly6C^high (d) and F4/80⁺CD11b⁺Ly6C^low (c) cells. The bar graphs summarize the percentage of F4/80⁺CD11b⁺Ly6C^high and F4/80⁺CD11b⁺Ly6C^low monocytes in the control, LPS, and LPS plus ASC groups. Data represent the mean ± SEM of n = 5–6 mice per group. **p < 0.01, ***p < 0.001. ASC adipose-derived mesenchymal stem (stromal) cell, LPS lipopolysaccharide