Fig. 6

Expression of the ERS biomarker GRP78 and autophagy-related factors LC3A/B and Beclin 1 and IHC for LC3A/B in the urinary bladder at 4 days after IC induction (n = 5). a The protein expression of GRP78, LC-3A/B, and Beclin1, one indicator of ERS and two indicators of autophagy, was highest in the interstitial cystitis (IC) group and lowest in the normal controls (N), and significantly lower in the IC + urine-derived stem cell (USC) group than in the IC group. b A statistical chart of the relative optical density of GRP78/GAPDH in each group (n = 5). c A statistical chart of the relative optical density of LC3B/LC3A/GAPDH in each group (n = 5). d A statistical chart of the relative optical density of Beclin1/GAPDH in each group (n = 5). e–g The protein expression of the index of autophagy, LC3A/B, by immunohistochemistry in the urinary bladders of each group of animals. h Analytical results of LC3 score. *P < 0.05, versus the control group; ^# P < 0.05, versus the IC group