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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-β/catenin signaling pathway

Fig. 2

Decreased expression of RNF43 protein in GC stem-like cells. a Western blot assay of RNF43, RNF75, Cul4a, and Sox-2 protein in cancer cells and HGC-27 and N87 GC stem-like cells. b qPCR of RNF43 mRNA expression in cancer cells and CG stem-like cells. Results represent mean ± SD of three independent experiments. c Representative images of primary tumorsphere cells (upper, HSC034; bottom, HSC035). Most spheres were larger than 100 μm in diameter after 7 days of culture. P passage. d Western blot assay of RNF43 and Sox-2 protein in primary tumorsphere cells and corresponding cancer and adjacent normal tissues. e qPCR of RNF43 mRNA expression in primary tumorsphere cells and corresponding cancer and adjacent normal tissues. Results represent mean ± SD of three independent experiments. *means Statistically significant (P < 0.05); **means Statistically significant (P < 0.01)

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Stem Cell Research & Therapy

ISSN: 1757-6512