Fig. 3

RNF43 OE affects proliferation and chemoresistance of GC cells through drug-induced apoptosis. a RNF43-overexpressing and control cells were treated with 5-Fu and oxaliplatin in different concentrations, and then cell viability was determined by CCK-8 assay. Results were normalized against the blank group (mean ± SD, n = 3). b CCK8 assay was performed to measure cell proliferation at the indicated times. c Cell apoptosis induced by 5-Fu and oxaliplatin was evaluated using Annexin V/propidium iodide-double staining. Early and late apoptotic cells were combined as Annexin V-positive cells that were considered criterion to calculate the percentage of cell apoptosis. 5-Fu 5-fluorouracil, OE overexpression