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Table 1 Primer sequences

From: The crosstalk between vascular MSCs and inflammatory mediators determines the pro-calcific remodelling of human atherosclerotic aneurysm

Gene

Primer sequence

α-SMA

FWD: ACCTTTGGCTTGGCTTGTCA

REV: GGAAGCTTTAGGGTCGCTGG

BMP-2

FWD: TGTCTTCTAGCGTTGCTGCT

REV: CAACTCGAACTCGCTCAGGA

CD31

FWD: CACAGATGAGAACCACGCCT

REV: GGCCCCTCAGAAGACAACAT

CD146

FWD: CTCACTCGGACGTCAGACAC

REV: AACACAGTGGGCGCTATGAA

GAPDH

FWD: AATGGGCAGCCGTTAGGAAA

REV: AGGAGAAATCGGGCCAGCTA

IL-1β

FWD: TGAGCTCGCCAGTGAAATGA

REV: AGATTCGTAGCTGGATGCCG

IL-10

FWD: GGGGCTTCCTAACTGCTACA

REV: TAGGGGAATCCCTCCGAGAC

MMP-9

FWD: GAACCAATCTCACCGACAG

REV: GCCACCCGAGTGTAACCAT

PPAR-γ

FWD: GTGGTAGGTAAGGAAGGGGC

REV: GGCTGACTCTCGTTTGAGAA

OPN

FWD: AGGCATCACCTGTGCCATAC

REV: GTCCAAGCTTCTGGGGACAA

OCN

FWD: CACCGAGACACCATGAGAGC

REV: CTGCTTGGACACAAAGGCTGC

TNF-α

FWD: CAGGGACCTCTCTCTAATCA

REV: TTGAGGGTTTGCTACAACAT

  1. α-SMA alpha-smooth muscle actin, BMP-2 bone morphogenetic protein-2, CD cluster of differentiation, GAPDH glyceraldehyde 3-phosphate dehydrogenase, IL interleukin, MMP-9 matrix metalloproteinase-9, PPAR-γ peroxisome proliferation activated gamma, OPN osteopontin, OCN osteocalcin, TNF-α tumour necrosis factor alpha
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Stem Cell Research & Therapy

ISSN: 1757-6512