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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton’s jelly and bone marrow-derived mesenchymal stem cells

Fig. 6

The role of sFRP4 and WISP1 in the adipogenic and osteogenic differentiation of WJ-MSCs. Graph a depicts the relative expression (mean + 1 standard deviation) of sFRP4 and WISP1 gene expression in WJ-MSCs at different time points during adipogenic and osteogenic differentiation respectively. Graph b depicts the relative expression (mean + 1 standard deviation) of the adipocyte-associated genes PPARG and CEBPA following induction of adipogenic differentiation of WJ-MSCs in the absence or presence of 20nM rh-sFRP4. Graph c shows the relative expression (mean + 1 standard deviation) of the osteocyte-associated genes RUNX2, ALP and OSC following induction of osteogenic differentiation of WJ-MSCs in the absence or presence of 50 ng/ml rh-WISP1. In all cases, evaluation of gene expression was performed by means of real-time RT-PCR using GAPDH as control. Comparisons in gene expression between different time points and between treated and untreated cultures were performed by means of the non-parametric Mann–Whitney U test. Abbreviations: rh-sFRP4 recombinant human secreted frizzled-related protein 4, rh-WISP1 recombinant human WNT-induced secreted protein 1, WJ-MSCs Wharton’s jelly-mesenchymal stem cells

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