Skip to main content
Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton’s jelly and bone marrow-derived mesenchymal stem cells

Fig. 7

The hematopoiesis-supporting capacity of WJ-MSCs and BM-MSCs. Confluent stromal layers from WJ-MSCs (n = 6) and BM-MSCs (n = 6) at P2 grown in 25cm2 flasks, were irradiated and recharged with normal allogeneic BM-derived (a) or UCB-derived (b) CD34+ cells. At weekly intervals for a total of 3 weeks, cultures were fed by demi-depopulation and the non-adherent cells were assayed for clonogenic progenitor cells in methylcellulose culture medium. The graphs depict the number (mean ± 1 standard deviation) of total colony-forming units (CFU), erythroid colony-forming units (CFU-E) and total granulocyte-macrophage colony-forming units (CFU-GM) time course obtained from the non-adherent cell fraction of recharged WJ-MSC or BM-MSC cultures. Comparison of mean colony numbers over the 3-week culture between WJ- and BM-MSC feeder layers was performed by the two-way analysis of variance and the P values are depicted. Abbreviations: BM-MSCs bone marrow-mesenchymal stem cells, UCB umbilical cord blood, WJ-MSCs Wharton’s jelly-mesenchymal stem cells

Back to article page