Fig. 3
From: Quiescence of human muscle stem cells is favored by culture on natural biopolymeric films
Pax7 and myogenin expression in SCs cultured on biopolymeric films. a Fluorescent labeling of actin, Pax7, myogenin (MyoG) and nucleus (Hoechst) at D3 on EDC10 and EDC70 films in comparison with plastic. Scale bar: 50 μm. b Pax7 and c MyoG expression were quantified by immunofluorescence at D1 and D3 in GM. Data from three independent experiments (n = 100 cells minimum per experimental condition). *p < 0.05, ***p < 0.001. d Western blot analysis of Pax7 and MyoG expression and semi-quantitative determination of e Pax7 and f MyoG expression as compared with plastic control (the condition “plastic D1” was set to 1). Results of D6 and D10 are only given for the culture on EDC10 films because cell confluence was reached on EDC70 films and plastic as soon as day 3; for these conditions, D6 and D10 are thus referred to as “not applicable” (NA). Data are mean ± SD from three independent experiments. D1, D3, D6, D10 days 1, 3, 6, 10