Fig. 4

Absence of SC proliferation on EDC10 films up to 10 days. a Quantification of SC proliferation by EdU assay: for D1, D3, D6 and D10 on EDC10 films and only for D1 and D3 on plastic, because confluence was reached (referred as “not applicable” (NA)). Data are box plots from three independent experiments (n = 200 cells in total per experimental condition). ***p < 0.001. b FACS analysis of the cell cycle at D3, D6 and D10 on EDC10 films and at D1 on plastic. Data plotted from two independent experiments (n = 5000 cells per condition). c Western blot of cyclin D1 and semi-quantitative analysis. The condition “plastic D1” was taken as the reference (value set to 1). Data are mean ± SD from three independent experiments. D1, D3, D6, D10 days 1, 3, 6, 10, EdU 5-ethynyl-2′-deoxyuridine