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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Quiescence of human muscle stem cells is favored by culture on natural biopolymeric films

Fig. 5

Reactivation of cells cultured on EDC10 films. a Schematic of experiment where SCs were precultured on EDC10 films or plastic for 3 days and then replated onto plastic. b Proliferation was assessed by EdU incorporation and c differentiation by the number of MHC+ cells after 3 days in DM. Data are mean ± SD from two different experiments (n = 50 cells per condition in each experiment). d Schematic of experiment where SCs were precultured either on EDC10 films or plastic for 3 days. SCs were then replated on EDC10 films and plastic for another 3 days. The cells were fixed and stained for actin and Pax7. e Representative images of actin staining. Scale bar: 50 μm. f Number of Pax7-positive cells: after the preculture (at D0), on EDC10 films and plastic, after replating for 3 days and depending on the preculture conditions (EDC10 films or plastic). Data are mean ± SD from three independent experiments (n = 100 cells). ***p < 0.001. D–3, D0, D3, days –3, 0, 3, EdU 5-ethynyl-2′-deoxyuridine, MHC skeletal myosin heavy chain

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