Fig. 1
From: High quality clinical grade human embryonic stem cell lines derived from fresh discarded embryos
a Derivation of human embryonic stem cell lines MAN lines 10–16 from surplus fresh IVF embryos. The derivation of human embryonic stem cell line MAN13 from (a) surplus fresh IVF embryos donated on day 4 post-fertilisation, which gave rise to (b) a blastocyst on day 6 of grade BL3Dc, with no discernible inner cell mass (two different focal planes through the blastocyst, bi and bii). Following plating onto hDF feeder cells, an outgrowth was established (c) and passaged (passage 1, P1) (d) in order to establish MAN13 at P5 – P12 (e- g), which could be maintained on a different line of hDF feeder cells (f). b The derivation of human embryonic stem cell lines MAN10–12 and MAN 14–16 on hDF feeder cells. Arrows indicate blastocysts used in derivation