Fig. 3
From: High quality clinical grade human embryonic stem cell lines derived from fresh discarded embryos
Immunostaining of in vitro differentiated cells from MAN lines 10–16. hESCs were subjected to in vitro differentiation via embryoid body formation and each line analysed by immunocytochemistry for two markers of each germ cell layer (green) with DAPI (blue) as a counterstain. Ectoderm markers: β-tubulin III and neurofilament; mesoderm markers alpha-smooth muscle actin (alpha-SMA) and vimentin; endoderm markers: GATA6 and FOXA2. Scale bars represent 100uM