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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: High quality clinical grade human embryonic stem cell lines derived from fresh discarded embryos

Fig. 4

Histological sections of teratomas derived from MAN lines 13–16. The teratomas were formed by subcutaneous injection of MAN13–16 cells under the kidney capsule of a SCID mouse. Stained sections show hematoxylin and eosin staining (A-L) of structures representative of the three germ layers: endoderm (A, D, G, J), mesoderm (B, E, H, K) and ectoderm (C, F, I, L) for MAN13 (A-C), MAN14 (D-F), MAN15 (G-I) and MAN16 (J-L). A, Kidney from endoderm; B, smooth muscle from mesoderm; C, retinal pigment epithelium from ectoderm; D, oesophagus from endoderm; E, artery from mesoderm; F, primitive neuroepithelium from ectoderm; G, intestine from endoderm; H, skeletal muscle from mesoderm; I, neural epithelium from ectoderm; J, sebaceous gland from endoderm; K, smooth muscle from mesoderm; L, skin tissue from ectoderm. a-d Alcian blue-fast red staining showing cartilage (mesoderm) in (a) MAN13, (b) MAN14, (c) MAN15 and (d) MAN16. Scale bars represent 100uM

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