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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation

Fig. 4

In-vitro angiogenesis-related assays. Freshly isolated MPCs as well as HUVECs revealed substantial Ac-LDL uptake, showing green fluorescence in most adherent cells. P2-MSCs showed no fluorescence (a). Quantitative evaluation (percentage of fluorescent areas) confirmed the observation (b). MPC transendothelial migration ability was reported in the presence of either FBS gradient or SDF-1β chemoattraction with a number of migrated cells on the outer surface of culture inserts (c, blue–violet). Conversely, few migrating P2-MSCs were reported exclusively under FBS gradient and only in part of the samples, compromising statistical significance (d) (“n.s.”, p > 0.05). Neither MPCs nor P2-MSCs were able to form CLS in capillary-like tube formation assay (e, f, g). Ac-LDL acetylated-low density lipoprotein, FBS fetal bovine serum, HUVEC human umbilical vein endothelial cell, MPC mesangiogenic progenitor cell, MSC mesenchymal stromal cell, N.D. not detected, SDF-1β stromal cell-derived factor 1 beta (Color figure online)

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