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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Exosomes as potential alternatives to stem cell therapy for intervertebral disc degeneration: in-vitro study on exosomes in interaction of nucleus pulposus cells and bone marrow mesenchymal stem cells

Fig. 6

Comparison of gene expression in BM-MSCs after coculture with NPCs and after stimulation by NPC exosomes. a Schematic diagram of experimental groups. NPCs and BM-MSCs were cocultured by an indirect method using a transwell system. NPCs were cultured in the upper chamber and BM-MSCs in the lower chamber to coculture for 14 days. NPC exosomes alone were added to BM-MSCs in the Exo group. BM-MSCs without treatment were taken as the control group. b After 14 days of treatment, qRT-PCR detected relative mRNA expression in BM-MSCs. Expression levels of ACAN (P < 0.01), SOX-9 (P < 0.05), COL2A1 (P < 0.01), HIF-1α (P < 0.01) and CA12 (P < 0.05), but not KRT19 (P > 0.05), mRNA were significantly higher in the Exo group than the coculture group. *P < 0.05, **P < 0.01, ***P < 0.001. BM-MSC bone marrow mesenchymal stem cell, Exo exosomes, NPC nucleus pulposus cell

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