Fig. 8

a Western blot analysis using antibodies specific to phospho-ERK1/2 and phospho-MEK in MM1 and MM4 cells stimulated with EGF in the presence or absence of AZ628 or sorafenib. α-tubulin was used to normalize protein loading. Representative immunoblots are reported. b Data derived from densitometric analysis of at least three independent experiments, using MM1 (left) and MM4 (right) cells. Data expressed as mean ± SEM of the percentage values of the EGF-induced ERK1/2 and MEK phosphorylation, set as 100%. *p <0.05, **p <0.01 vs EGF-stimulated cells. EGF endothelial growth factor