Protein | ADSC monoculture (pg/ml) | Respective BRCA monoculture (pg/ml) | Co-culture (pg/ml) | Fold-change for ADSC (monoculture/co-culture) | Fold-change for respective BRCA (monoculture/co-culture) |
---|---|---|---|---|---|
Co-culture of ADSCs and EVSA-T cell line | |||||
CCL2 | 1755 (65) | ND | 1421 (108) | 0.9 → | NA ↑↑ |
EMMPRIN | 594 (46) | 208 (7.6) | 828 (37) | 1.4 → | 4.0 ↑ |
HGF | 1803 (159) | ND | 1104 (155) | 0.6 → | NA ↑↑ |
IL-6 | 62 (11) | ND | 53 (7.1) | 0.9 → | NA ↑↑ |
IL-7 | 8.4 (6.3) | ND | 14 (6.8) | 1.7 → | NA ↑ |
IL-8 | 4261 (673) | ND | 3818 (665) | 0.9 → | NA ↑↑ |
MMP-1 | 165,407 (32,918) | n.d | 80,345 (3137) | 0.5 → | NA ↑↑ |
MMP-2 | 61,271 (3320) | ND | 66,795 (5514) | 1.1 → | NA ↑↑ |
MMP-3 | 8274 (355) | 23 (1.5) | 5356 (142) | 0.7 → | 233 ↑↑ |
MMP-7 | 208 (40) | ND | 156 (29) | 0.8 → | NA ↑ |
MMP-9 | 82 (5.4) | 32 (2.8) | 70 (2.9) | 0.9 → | 2.2 ↑ |
MMP-10 | 385 (23) | 21 (2.7) | 258 (6.8) | 0.7 → | 12 ↑ |
VEGF | 47 (0.0) | ND | 38 (4.6) | 0.8 → | NA ↑↑ |
Co-culture of ADSCs and MCF-7 cell line | |||||
CCL2 | 2004 (99) | ND | 1007 (75) | 0.5 → | NA ↑↑ |
CCL3 | 16 (0.7) | 14 (1.6) | 17 (2.0) | 1.1 → | 1.2 → |
CCL5 | 5.1 (3.4) | 12 (7.6) | 4.9 (2.6) | 1.0 → | 0.4 ↓ |
CXCL10 | 2.3 (0.1) | 1.9 (0.1) | 2.1 (0.2) | 0.9 → | 1.1 → |
EMMRPIN | 768 (34) | 551 (28) | 1073 (57) | 1.4 → | 2.0 → |
Eotaxin | 1.0 (0.1) | 1.2 (0.2) | 0.9 (0.1) | 0.8 → | 0.8 → |
G-CSF | 91 (9.7) | 29 (5.2) | 55 (8.3) | 0.6 → | 1.9 → |
GM-CSF | 1.7 (0.1) | 1.0 (0.1) | 1.3 (0.1) | 0.7 → | 1.3 → |
HGF | 2095 (244) | ND | 1488 (67) | 0.7 → | NA ↑↑ |
IL-4 | 18 (1.0) | 12 (0.6) | 16 (1.3) | 0.9 → | 1.3 → |
IL-5 | 2.8 (0.1) | 2.8 (0.2) | 2.9 (0.3) | 1.0 → | 1.0 → |
IL-6 | 160 (20) | ND | 160 (23) | 1.0 → | NA ↑↑ |
IL-8 | 5330 (633) | ND | 2982 (367) | 0.6 → | NA ↑↑ |
IL-10 | 4.6 (0.5) | 4.0 (0.4) | 4.9 (0.4) | 1.1 → | 1.2 → |
IL-12 | 11.7 (4.2) | ND | 10.6 (5.1) | 0.9 → | NA ↑ |
IL-13 | 10 (1.0) | 15 (0.5) | 20 (1.7) | 2.0 ↑ | 1.3 → |
IL-1Ra | 24 (1.7) | 24 (1.1) | 25 (1.5) | 1.0 → | 1.0 → |
INF-α | 80 (6.5) | ND | 46 (7.3) | 0.6 → | NA ↑↑ |
MMP-1 | 85,387 (18,267) | 30 (5.4) | 17,881 (1437) | 0.2 → | 596 ↑↑ |
MMP-2 | 51,127 (3291) | ND | 74,585 (6060) | 1.5 → | NA ↑↑ |
MMP-3 | 3514 (281) | 40 (0.0) | 3,905 (173) | 1.1 → | 98 ↑↑ |
MMP-9 | 112 (7,6) | 112 (3.2) | 99 (4.0) | 0.9 → | 0.9 → |
MMP-10 | 193 (76) | 41 (3.9) | 236 (6.7) | 1.2 → | 5.8 ↑ |
VEGF | 44 (4.7) | ND | 48 (6.1) | 1.1 → | NA ↑↑ |
Co-culture of ADSCs and MDA-MB-231 cell line | |||||
CCL2 | 965 (140) | 2.4 (0.0) | 949 (162) | 1.0 → | 395 ↑↑ |
EMMPRIN | 496 (20) | 434 (30) | 882 (53) | 1.8 → | 2.0 → |
HGF | 889 (206) | 9.2 (4.9) | 708 (129) | 0.8 → | 77 ↑↑ |
IL-4 | 1.7 (1.0) | 1.4 (0.0) | 0.9 (0.6) | 0.5 → | 0.6 → |
IL-6 | 199 (32) | 80 (3.9) | 207 (36) | 1.0 → | 2.6 ↑ |
IL-7 | 22 (2.6) | 17 (2.0) | 15 (6.4) | 0.7 → | 0.9 → |
IL-8 | 3238 (528) | 350 (32) | 2836 (474) | 0.9 → | 8.1 ↑ |
MMP-1 | 58,500 (5616) | 5637 (414) | 32,448 (3927) | 0.6 → | 5.8 ↑ |
MMP-2 | 17,767 (1796) | ND | 18,290 (1907) | 1.0 → | NA ↑↑ |
MMP-3 | 2185 (79) | 196 (11) | 1994 (118) | 0.9 → | 10 ↑ |
MMP-9 | 105 (4.0) | 93 (6.1) | 101 (5.8) | 1.0 → | 1.1 → |
MMP-10 | 160 (7.0) | 50 (3.1) | 151 (11) | 0.9 → | 3.0 ↑ |
VEGF | 22 (12) | 24 (0.0) | 25 (9.2) | 1.1 → | 1.0 → |
Co-culture of ADSCs and SK-BR-3 cell line | |||||
bFGF | 4.8 (0.0) | ND | ND | ||
CCL2 | 1200 (134) | 24 (5.8) | 699 (92) | 0.6 → | 29 ↑↑ |
EMMPRIN | 566 (22) | 456 (13) | 1030 (50) | 1.8 → | 2.3 → |
HGF | 975 (159) | ND | 537 (94) | 0.6 → | NA ↑↑ |
IL-4 | 1.2 (0.9) | ND | 0.6 (0.6) | 0.5 → | NA ↑↑ |
IL-6 | 175 (20) | 4.3 (1.6) | 145 (15) | 0.8 → | 34 ↑↑ |
IL-7 | 13 (8.1) | ND | 6.9 (5.6) | 0.5 ↓ | NA ↑↑ |
IL-8 | 3581 (263) | 66 (15) | 2540 (337) | 0.7 → | 39 ↑↑ |
IL-1Ra | 20 (0.0) | ND | ND | NA ↓ | NA → |
INF-α | 30 (7.5) | ND | ND | NA ↓ | NA → |
MMP-1 | 62,844 (4604) | 29 (2.0) | 37,601 (2334) | 0.6 → | 1297 ↑ |
MMP-2 | 42,296 (2367) | ND | 59,467 (4706) | 1.4 → | NA ↑↑ |
MMP-3 | 3472 (316) | 48 (3.0) | 3261 (251) | 0.9 → | 68 ↑↑ |
MMP-9 | 111 (8.5) | 255 (14) | 128 (10) | 1.2 → | 0.5 → |
MMP-10 | 231 (15) | 33 (13) | 219 (10) | 1.0 → | 6.4 ↑ |
VEGF | 24 (9.7) | ND | 23 (7.9) | 1.0 → | NA ↑↑ |
Co-culture of ADSCs and ZR75-30 cell line | |||||
CCL2 | 3603 (375) | 112 (11) | 3137 (212) | 0.9 → | 28 ↑↑ |
CCL3 | 21 (2.1) | 16 (1.4) | 18 (2.0) | 0.9 → | 1.2 → |
CCL4 | 2.2 (0.7) | ND | 2.7 (0.0) | 1.2 → | NA ↑ |
CCL5 | 7.8 (4.1) | 6.4 (2.2) | 3.1 (1.2) | 0.4 ↓ | 0.5 → |
CXCL10 | 2.9 (0.3) | 2.1 (0.1) | 2.3 (0.3) | 0.8 → | 1.1 → |
EMMPRIN | 819 (40) | 21 (2.5) | 594 (4.9) | 0.7 → | 28 ↑↑ |
Eotaxin | 1.2 (0.3) | 1.3 (0.2) | 0.9 (0.1) | 0.8 → | 0.7 → |
G-CSF | 85 (15) | 32 (8.5) | 54 (14) | 0.6 → | 1.7 → |
GM-CSF | 2.2 (0.4) | 1.1 (0.0) | 1.7 (0.2) | 0.8 → | 1.5 → |
HGF | 4672 (557) | ND | 2589 (239) | 0.6 → | NA ↑↑ |
IL-4 | 21 (3.0) | 13 (0.0) | 18 (1.6) | 0.9 → | 1.4 → |
IL-5 | 3.0 (0.2) | 2.8 (0.1) | 3.0 (0.3) | 1.0 → | 1.1 → |
IL-6 | 186 (42) | ND | 151 (10) | 0.8 → | NA ↑↑ |
IL-7 | 24 (11) | ND | ND | NA ↓↓ | NA ↓↓ |
IL-8 | 4686 (714) | 33 (7.8) | 2578 (316) | 0.6 → | 78 ↑↑ |
IL-10 | 5.2 (0.3) | 4.2 (0.3) | 5.0 (0.8) | 1.0 → | 1.2 → |
IL-12 | 20 (5.8) | ND | 13 (8.5) | 0.7 → | NA ↑↑ |
IL-13 | 23 (2.8) | 16 (0.9) | 20 (1.7) | 0.9 → | 1.3 → |
IL-1Ra | 28 (3.6) | 26 (1.4) | 26 (3.2) | 0.9 → | 1.0 → |
INF-α | 116 (9.7) | ND | 90 (6.3) | ||
INF-γ | 1.2 (0.8) | 0.2 (0.2) | 2.1 (0.0) | 1.8 → | 11 ↑ |
MMP-1 | 116,547 (31,612) | 18 (0.6) | 43,964 (2942) | 0.4 ↓ | 2442 ↑↑ |
MMP-2 | 121,997 (6892) | ND | 131,537 (2758) | 1.1 → | NA ↑↑ |
MMP-3 | 5264 (444) | 43 (2.0) | 2665 (210) | 0.5 → | 62 ↑↑ |
MMP-9 | 110 (4.2) | 89 (5.3) | 106 (6.5) | 1.0 → | 1.2 → |
MMP-10 | 292 (17) | 39 (2.6) | 173 (6.6) | 0.6 → | 4.4 ↑ |
VEGFA | 56 (8.4) | ND | 49 (5.0) | ||
Co-culture of ADSCs and primary BRCAs | |||||
bFGF | 8.7 (0.0) | 60 (9.2) | 46 (7.7) | 5.3 ↑ | 0.8 → |
CCL2 | 5241 | 24,396 (2324) | 35,559 (1471) | 16 ↑↑ | 1.4 → |
CCL3 | ND | 135 | 126 | NA ↑↑ | 1.0 → |
CCL4 | ND | 37 | 48 | NA ↑↑ | 1.3 → |
CCL5 | 37 (11) | 136 (9.2) | 110 (14) | 3.0 ↑ | 0.8 → |
CXCL-9 | ND | 271 (9.2) | 553 (68) | 20 ↑ | 2.0 → |
CXCL-10 | 6.5 (0.4) | 572 (9.2) | 89 (15) | 13.6 ↑ | 6.4 ↓ |
EGF | ND | 71 (9.2) | 49 (22) | NA ↑↑ | 0.7 → |
EMMRPIN | 104 (12) | 1908 (281) | 1949 (122) | 19 ↑↑ | 1.0 → |
Eotaxin | 1.1 (0.6) | 4.2 (9.2) | 6.5 (1.4) | 5.8 ↑ | 1.6 → |
G-CSF | ND | 1604 (9.2) | 1396 (142) | NA ↑ | 0.8 → |
GM-CSF | 6.8 (0.3) | 23 (9.2) | 37 (0.7) | 5.4 ↑ | 1.5 → |
HGF | 1740 (99) | 398 (9.2) | 1254 (99) | 0.7 → | 3.2 ↑ |
IL-1b | ND | 84 (9.2) | 75 (22) | NA → | 0.9 → |
IL-2 | ND | 10 (9.2) | 7.3 (2.6) | NA → | 0.7 → |
IL-4 | 40 (4.2) | 105 (9.2) | 121 (6.3) | 3.0 ↑ | 1.2 → |
IL-5 | ND | 5.1 (9.2) | 6.9 (1.7) | NA ↑ | 1.3 → |
IL-6 | 1222 (114) | 7768 (1007) | 12,121 (409) | 20 ↑↑ | 1.5 → |
IL-7 | 45 (37) | 248 (9.2) | 251 (32) | 5.6 ↑ | 1.0 → |
IL-8 | 3224 (150) | 17,925 (2899) | 34,099 (1586) | 11 ↑↑ | 1.9 → |
IL-10 | 22 (1.7) | 62 (9.2) | 56 (4.6) | 2.6 ↑ | 0.9 → |
IL-12 | 47 (5.2) | 179 (9.2) | 207 (11) | 4.4 ↑ | 1.2 → |
IL-13 | 23 (4.1) | 57 (9.2) | 63 (11) | 2.7 ↑ | 1.1 → |
IL-15 | 48 (22) | 363 (9.2) | 235 (49) | 4.9 ↑ | 0.7 → |
IL-17 | ND | 22 | 27 | NA ↑ | 1.2 → |
IL-2R | 90 (65) | 398 (9.2) | 425 (113) | 4.7 ↑ | 1.0 → |
INF-α | 172 (16) | 950 (9.2) | 1186 (35) | 6.9 ↑ | 1.3 → |
INF-y | 4.7 (0.0) | 24 (9.2) | 20 (6.4) | 4.4 ↑ | 0.8 → |
MMP-1 | 8045 (483) | 3606 (509) | 12,850 (1750) | 1.6 → | 3.6 ↑ |
MMP-2 | 75,480 (3780) | 17,192 (2131) | 108,563 (4932) | 1.4 → | 6.3 ↑ |
MMP-3 | 1218 (51) | 757 (145) | 5005 (395) | 4.1 ↑ | 6.6 ↑ |
MMP-9 | 94 (7.4) | 873 (107) | 852 (50) | 9.0 ↑ | 1.0 → |
MMP-10 | 82 (2.5) | 542 (70) | 857 (54) | 11 ↑↑ | 1.6 → |
TNF-α | 2.0 (2.6) | 18 (9.2) | 20 (4.2) | 9.8 ↑ | 1.1 → |
VEGF | 131 (16) | 518 (9.2) | 629 (32) | 4.8 ↑ | 1.2 → |