Fig. 4

TAF-iPS cell lines show efficient differentiation capacity into haematopoietic and neural cell lineages. a Representative FACS plots and percentage of haematopoietic cell outputs following differentiation of five TAF-iPS cell lines, five CB-iPS cell lines, and four hES cell lines (HUES-3, HUES-6, H1, H9). Each dot represents a distinct pluripotent stem cell line. b Colony forming unit (CFU) counts per 10,000 plated cells. CFU-G granulocyte, CFU-M macrophage, CFU-GM granulocyte/macrophage, CFU-GEMM granulocyte/erythroid/macrophage, BFU-E burst forming unit—erythroid. c Representative images from cytospin preparation of CFU-G, CFU-M, and BFU-E stained with May–Grünwald–Giemsa. d Benzidine staining demonstrating haemoglobinized erythroid cells. e Representative immunofluorescent images of TUJ1 staining in TAF-iPS and CB-iPS cell cultures after neural induction. f, g Quantitative gene expression analyses of eight neural markers and non-neural marker COL3A1 after neural induction. Data represent an average of three to five independent experiments for each cell line and three technical replicates per sample, calculated as fold change expression relative to undifferentiated hES cell line, H9. h Immunofluorescent staining of TAF-iPS and CB-iPS cell lines after neural induction for TUJ1 and the non-neuronal marker TE7. Data represented as mean ± SEM. Statistical analysis was performed using Student’s t test. *p < 0.05. Scale bars: all 100 μm. CB-iPS cell cord blood-derived induced pluripotent stem cell, hES cell human embryonic stem cell, TAF-iPS cell term amniotic fluid-derived induced pluripotent stem cell