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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Generation of tooth–periodontium complex structures using high-odontogenic potential dental epithelium derived from mouse embryonic stem cells

Fig. 1

mES cell-derived epithelium. a Cell morphology. mES cells present a colony growth character, each clone with an obvious boundary. EBs are round balls. One day after attachment, cells began to climb out peripheral from the EBs (radial growth). A large number of epithelioid cells presented on day 3 and later. b qRT-PCR showed representative downregulation of mES cell-specific transcription factor (Oct4) and the expression of keratin epithelial marker k14 was upregulated on day 3. Expression of early ectoderm marker P63 and K18 was relative lower on day 3. FGF8 expression was up regulated on day 3 while BMP4 showed a high expression level. c Immunostaining of the day 3 cells for FGF8 and BMP4. BMP4 bone morphogenic protein 4, D day, EB embryoid body, FGF8 fibroblast growth factor 8, mESC mouse embryonic stem cell

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