Fig. 6

The effect of Wnt/β-catenin signaling inhibitor (DKK1) and Smad3 inhibitor (SIS3) on the expression of osteo-specific genes in BM-MSCs on PCL nanofiber scaffolds. a Western blotting was used to detect the expression of β-catenin in DKK1-treated BM-MSCs on PCL nanofiber scaffolds. b Western blotting was used to detect the activation of Smad3 in SIS3-treated BM-MSC on PCL nanofiber scaffolds. c-f Real-time PCR was performed to examine the expression of osteo-specific genes in DKK1 and/or SIS3-treated BM-MSCs on PCL nanofiber scaffolds. ^* P < 0.05; ^** P < 0.01; ^*** P < 0.001. BM-MSC human bone marrow-derived MSCs, DKK1 Wnt/β-catenin signaling inhibitor, SIS3 Smad3 signaling inhibitor