Fig. 4

The β-TCP scaffold was approximately 8 mm in diameter and 1.5 mm in thickness (a,b). The OM and iEC/UM sheets were cut into strips approximately 60 mm in length and 10 mm in width, then wrapped onto the β-TCP scaffold, thus generating the BP/β-TCP (c,d). SEM images of BP/β-TCP indicated that the cells from the cell sheet apparently migrated into the porous β-TCP scaffold and that rich ECM surrounded the scaffold (e,f). Scale bars = 500 μm (e) and 100 μm (f)