Fig. 6

H&E staining on the sectioned slices at 2 weeks showing that the plain β-TCP (a) and no-implantation group (b) had hardly any lumen formation; however, numerous lumens containing murine erythrocytes in the AP/β-TCP group (c) and BP/β-TCP group (d) were observed. Similar results were seen in the immunohistochemical staining of CD31 on the four groups: plain β-TCP (e), no-implantation group (f), AP/β-TCP (g), and BP/β-TCP group (h). Two magnified images in the BP/β-TCP group clearly show the lumens containing murine erythrocytes (scale bar = 100 μm). Quantitative results (i) show a significant difference in the vascular volume among the periosteum groups and nonperiosteum group (p < 0.05) by counting CD31-positive expressing lumens, while there was no significant difference between the BP/β-TCP and AP/β-TCP groups(p < 0.05). β-TCP beta-tricalcium phosphate, AP autologous periosteum, BP biomimetic periosteum