Fig. 4

Generation of iPS cell with retroviral GFP and OSKM transduction. a Gene expression in the CP, FM, FM3%4D, and FM8%4D groups by RT-PCR at day 7. b Western blot analysis of OCT4 and SOX2 expression at day 7. c Retroviral OSKM transduction cell morphology change: observation of retroviral GFP infection cell morphology (left row), retroviral OSKM infection cell morphology (middle row), and magnified image (right row). Scale bar = 100 μm. CP commercial plates, D days, FM flexible membranes, GFP green fluorescent protein, Retro-OSKM pMXs-OCT4, pMXs-SOX2, pMXs-KLF, pMXs-c-MYC