Table 1 Primers used for reverse-transcription polymerase chain reaction
Target | Primer | Sequence (5′ to 3′) |
|---|---|---|
Total OCT4 | F | GAGAAGGATGTGGTCCGAGTGTG |
Total OCT4 | R | CAGAGGAAAGGACACTGGTCCC |
Total SOX2 | F | AGAACCCCAAGATGCACAAC |
Total SOX2 | R | ATGTAGGTCTGCGAGCTGGT |
Total KLF4 | F | ACCCTGGGTCTTGAGGAAGT |
Total KLF4 | R | ACGATCGTCTTCCCCTCTTT |
Total c-MYC | F | CCTACCCTCTCAACGACAGC |
Total c-MYC | R | CTCTGACCTTTTGCCAGGAG |
Endo-OCT4 | F | GACAGGGGGAGGGGAGGAGCTAGG |
Endo-OCT4 | R | CTTCCCTCCAACCAGTTGCCCCAAAC |
Endo-SOX2 | F | GGGAAATGGGAGGGGTGCAAAAGAGG |
Endo-SOX2 | R | TTGCGTGAGTGTGGATGGGATTGGTG |
Endo-KLF4 | F | ACGATCGTGGCCCCGGAAAAGGACC |
Endo-KLF4 | R | TGATTGTAGTGCTTTCTGGCTGGGCTCC |
Endo-c-MYC | F | GCGTCCTGGGAAGGGAGATCCGGAGC |
Endo-c-MYC4 | R | TTGAGGGGCATCGTCGCGGGAGGCTG |
hTERT | F | CGGAAGAGTGTCTGGAGCAA |
hTERT | R | GGATGAAGCGGAGTCTGGA |
TDGF | F | TCCTTCTACGGACGGAACTG |
TDGF | R | AGAAATGCCTGAGGAAAGCA |
Nanog | F | CAAAGGCAAACAACCCACTT |
Nanog | R | ATTGTTCCAGGTCTGGTTGC |
pMXs-AS3200 | R | TTATCGTCGACCACTGTGCTGCTG |
pMXs-L3205 | R | CCCTTTTTCTGGAGACTAAATAAA |
Exo-hOCT4 | F | CCCCAGGGCCCCATTTTGGTACC |
Exo-hSOX2 | F | GGCACCCCTGGCATGGCTCTTGGCTC |
Exo-hKLF4 | F | ACGATCGTGGCCCCGGAAAAGGACC |
Exo-c-MYC | F | CAACAACCGAAAATGCACCAGCCCCAG |
hSOX17 | F | CGC TTT CAT GGT GTG GGC TAA GGA CG |
hSOX17 | R | TAG TTG GGG TGG TCC TGC ATG TGC TG |
hAFP | F | GAA TGC TGC AAA CTG ACC ACG CTG GAA C |
hAFP | R | TGG CAT TCA AGA GGG TTT TCA GTC TGG A |
hCK8 | F | CCT GGA AGG GCT GAC CGA CGA GAT CAA |
hCK8 | R | CTT CCC AGC CAG GCT CTG CAG CTC C |
hCK18 | F | AGC TCA ACG GGA TCC TGC TGC ACC TTG |
hCK18 | R | CAC TAT CCG GCG GGT GGT GGT CTT TTG |
hMAP2 | F | CAG GTG GCG GAC GTG TGA AAA TTG AGA GTG |
hMAP2 | R | CAC GCT GGA TCT GCC TGG GGA CTG TG |
hPAX6 | F | ACC CAT TAT CCA GAT GTG TTT GCC CGA G |
hPAX6 | R | ATG GTG AAG CTG GGC ATA GGC GGC AG |