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Table 1 Primers used for reverse-transcription polymerase chain reaction
| Target | Primer | Sequence (5′ to 3′) |
|---|---|---|
| Total OCT4 | F | GAGAAGGATGTGGTCCGAGTGTG |
| Total OCT4 | R | CAGAGGAAAGGACACTGGTCCC |
| Total SOX2 | F | AGAACCCCAAGATGCACAAC |
| Total SOX2 | R | ATGTAGGTCTGCGAGCTGGT |
| Total KLF4 | F | ACCCTGGGTCTTGAGGAAGT |
| Total KLF4 | R | ACGATCGTCTTCCCCTCTTT |
| Total c-MYC | F | CCTACCCTCTCAACGACAGC |
| Total c-MYC | R | CTCTGACCTTTTGCCAGGAG |
| Endo-OCT4 | F | GACAGGGGGAGGGGAGGAGCTAGG |
| Endo-OCT4 | R | CTTCCCTCCAACCAGTTGCCCCAAAC |
| Endo-SOX2 | F | GGGAAATGGGAGGGGTGCAAAAGAGG |
| Endo-SOX2 | R | TTGCGTGAGTGTGGATGGGATTGGTG |
| Endo-KLF4 | F | ACGATCGTGGCCCCGGAAAAGGACC |
| Endo-KLF4 | R | TGATTGTAGTGCTTTCTGGCTGGGCTCC |
| Endo-c-MYC | F | GCGTCCTGGGAAGGGAGATCCGGAGC |
| Endo-c-MYC4 | R | TTGAGGGGCATCGTCGCGGGAGGCTG |
| hTERT | F | CGGAAGAGTGTCTGGAGCAA |
| hTERT | R | GGATGAAGCGGAGTCTGGA |
| TDGF | F | TCCTTCTACGGACGGAACTG |
| TDGF | R | AGAAATGCCTGAGGAAAGCA |
| Nanog | F | CAAAGGCAAACAACCCACTT |
| Nanog | R | ATTGTTCCAGGTCTGGTTGC |
| pMXs-AS3200 | R | TTATCGTCGACCACTGTGCTGCTG |
| pMXs-L3205 | R | CCCTTTTTCTGGAGACTAAATAAA |
| Exo-hOCT4 | F | CCCCAGGGCCCCATTTTGGTACC |
| Exo-hSOX2 | F | GGCACCCCTGGCATGGCTCTTGGCTC |
| Exo-hKLF4 | F | ACGATCGTGGCCCCGGAAAAGGACC |
| Exo-c-MYC | F | CAACAACCGAAAATGCACCAGCCCCAG |
| hSOX17 | F | CGC TTT CAT GGT GTG GGC TAA GGA CG |
| hSOX17 | R | TAG TTG GGG TGG TCC TGC ATG TGC TG |
| hAFP | F | GAA TGC TGC AAA CTG ACC ACG CTG GAA C |
| hAFP | R | TGG CAT TCA AGA GGG TTT TCA GTC TGG A |
| hCK8 | F | CCT GGA AGG GCT GAC CGA CGA GAT CAA |
| hCK8 | R | CTT CCC AGC CAG GCT CTG CAG CTC C |
| hCK18 | F | AGC TCA ACG GGA TCC TGC TGC ACC TTG |
| hCK18 | R | CAC TAT CCG GCG GGT GGT GGT CTT TTG |
| hMAP2 | F | CAG GTG GCG GAC GTG TGA AAA TTG AGA GTG |
| hMAP2 | R | CAC GCT GGA TCT GCC TGG GGA CTG TG |
| hPAX6 | F | ACC CAT TAT CCA GAT GTG TTT GCC CGA G |
| hPAX6 | R | ATG GTG AAG CTG GGC ATA GGC GGC AG |
