Fig. 2

Scheme of the present study. a Two million passage 2 human synovial MSCs were suspended in glucose acetated Ringer’s solution or complete human serum. The cells were preserved at 4 °C, 13 °C, or 37 °C for 48 h, and then the cells were evaluated. b The cells were analyzed at time 0 and 48 h after preservation for live/dead, apoptosis, surface marker and metabolic assays. Then, the cells were cultured for 14 days for proliferation and colony forming abilities. The cells were also pelleted and cultured in chondrogenic medium for 21 days. Furthermore, the cells were cultured for 14 days to make cell colonies, then cultured in adipogenic medium and in calcification medium for 21 days