Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation

Table 1 Important components of SVF, respective sizes, and surface markers

Cell types of the SVF	Cell size range [in µm]*	Molecular markers^# [1, 26, 29]
Cell types of the SVF	Cell size range [in µm]*	Positive	Negative
ADSC	~10–25 μm and reported up to 200+ μm in culture [34,35,36]	CD34, CD73, CD13, CD90, CD105, CD29	CD31, CD45, CD144
EPC	~7–8 μm (smallest defined) [37]	CD34, CD31, CD133, CD146	CD45
EC	~10–30 μm [38]	CD31, FVIII	CD34
T regulatory cells	~7–12 μm [39, 40]	CD4, CD25, Foxp3, CD8	–
Macrophages	~20 μm [41]	CD45, CD14, CD34, CD206	–
Smooth muscle cells	~3–20 μm in width and 20–500 μm in length [42, 43]	Smooth muscle actin (SMA)	–
Pericytes	Up to ~70 μm in length [44]	CD146, CD90, CD73, CD44, CD29, CD13	CD34, CD45, CD56
Pre-adipocytes	~10 μm [45]	CD34	CD45, CD31, CD146

*Diameter; unless mentioned otherwise
The Table captures the approximate range of cell sizes as reported in different studies [34,35,36,37,38,39,40,41,42,43,44,45] and provides an overview of surface antigens for the respective cell type [1, 26, 29]. ^# Includes surface or CD markers, cytoplasmic and nuclear factors
ADSC adipose-derived stem/stromal cells, EC endothelial cells, EPC endothelial precursor cells, SVF stromal vascular fraction