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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Bone mesenchymal stem cells ameliorate ischemia/reperfusion-induced damage in renal epithelial cells via microRNA-223

Fig. 3

Expression of growth factors, chemokines, and antiapoptosis factors was enhanced by coculture with mesenchymal stem cells (MSCs), while expression of proapoptosis factors was suppressed. Renal tubular epithelial cells (RTECs) were treated with hypoxia/reoxygenation (HR) stimulation and then cocultured with MSCs or hypoxia-pretreated MSCs (htMSCs) in a double-chamber. Cells were harvested at 48 h. a The concentrations of hepatocyte growth factor (HGF), insulin-like growth factor-1 (IGF-1), transforming growth factor beta (TGF-β), and vascular endothelial growth factor (VEGF) in coculture supernatants were measured by ELISA. b Expression of B-cell lymphoma-2 (Bcl-2), B-cell lymphoma-XL (Bcl-XL), cysteine protease protein-1 (caspase-1), and cysteine protease protein-3 (caspase-3) in RTECs was detected by qRT-PCR. c Representative images of Western blot assays for apoptosis-related indicators. a P < 0.05, versus the RTEC group; b P < 0.05, versus the HR RTEC group; c P < 0.05, versus the HR RTEC + MSC group. GAPDH glyceraldehyde-3-phosphate dehydrogenase

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