Fig. 6

Expression of growth factors, chemokines, and antiapoptosis factors was suppressed by coculture with mesenchymal stem cells (MSCs) when miR-223 was inhibited; however, expression of proapoptosis factors was enhanced. All renal tubular epithelial cells (RTECs) were treated with hypoxia/reoxygenation (HR) stimulation. The MSCs had been transfected in advance with a miR-223 inhibitor (miR-223 in). The RTECs and MSCs were then cocultured in a double-chamber. a The concentrations of hepatocyte growth factor (HGF), insulin-like growth factor-1 (IGF-1), transforming growth factor beta (TGF-β), and vascular endothelial growth factor (VEGF) in coculture supernatants were measured with ELISA. b Expression of B-cell lymphoma-2 (Bcl-2), B-cell lymphoma-XL (Bcl-XL), cysteine protease protein-1 (caspase-1), and cysteine protease protein-3 (caspase-3) in RTECs was detected by qRT-PCR. c Representative images of Western blot assays for apoptosis-related indicators. ^a P < 0.05, versus the RTEC + MSC group. GAPDH glyceraldehyde-3-phosphate dehydrogenase