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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Bone mesenchymal stem cells ameliorate ischemia/reperfusion-induced damage in renal epithelial cells via microRNA-223

Fig. 7

Expression of NLRP3 in renal tubular epithelial cells (RTECs) was upregulated after coculture with mesenchymal stem cells (MSCs), during which miR-223 was inhibited. All RTECs were treated with hypoxia/reoxygenation (HR) stimulation. The MSCs had been transfected in advance with a miR-223 inhibitor (miR-223 in). The RTECs and MSCs were then cocultured in a double-chamber. a Expression of NLR family-pyrin domain containing-3 (NLRP3) in RTECs was detected by qRT-PCR and Western blot assays. a P < 0.05, versus the RTEC + MSC group. b Representative images from the immunofluorescence assay for NLRP3 in RTECs. DAPI 4,6-diamino-2-phenyl indole, GAPDH glyceraldehyde-3-phosphate dehydrogenase

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Stem Cell Research & Therapy

ISSN: 1757-6512