Fig. 1

Characterization of EVs. a Representative graph of the intensity and hydrodynamic diameter of extracellular vesicle samples analyzed using the dynamic light scattering technique. Graph showing two populations of extracellular vesicles obtained from human adipose tissue-derived mesenchymal stromal cells, one of lower intensity and medium size, characteristic of exosomes, and another with greater intensity and average size, characteristic of microvesicles. b, c, and d Scanning electron microscopy of mesenchymal stromal cells. b Mesenchymal stromal cells before induction of cellular stress (fetal bovine serum deprivation), showing the presence of few extracellular vesicles. c Image obtained 12 hours after cellular stress induction, showing increase in the number of vesicles in the cell surface. d Higher magnification of image C, showing extracellular vesicles with some indicative sizes