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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Modulation of oxidative phosphorylation and redox homeostasis in mitochondrial NDUFS4 deficiency via mesenchymal stem cells

Fig. 5

a BN-PAGE with mitochondrial lysates from human and mouse fibroblasts cell lines shows no recovery of fully assembled complex I levels upon treatment with MSC supernatant. Complex II SDHA was used as loading control. b Complex I in-gel-activity assay demonstrates no improvement of CI activity upon treatment with MSC supernatant. c A complex I enzyme activity microplate assay kit was used to determine the complex I activity in mouse NDUFS4 KO and WT fibroblasts treated or untreated with supernatant from mMSCs. In accordance with BN-PAGE analysis, no significant increase in activity could be detected. Rate (mOD/min) = Absorbance 1 – Absorbance 2 / time (min). mMSC mouse mesenchymal stem cell, MSC mesenchymal stem cell, NDUFS4 NADH dehydrogenase ubiquinone Fe-S protein 4, WT wild type

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