Fig. 2

Microarray validation of selected transcripts by qRT-PCR analysis. Array results were validated choosing transcripts relevant for oxidative stress. Dark grey columns represent HUES3 cells and black columns represent HUES7 cells. Relative gene expression between CTR, untreated control cells, and 4 μM, 8 μM and 16 μM H₂O₂ conditions of 72 h treatment. H₂O₂ exposure induced a dose-response modification of oxidation-reduction processes genes: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3 (a), peroxiredoxin 2 (b), peroxiredoxin 5 (c) and cytochrome C oxidase assembly protein (d) were upregulated for treated cells. Upregulated genes related with sugars and sterol metabolism: aldo-keto reductase family 1, member A1 (e) and transmembrane 7 superfamily member 2 (f). Downregulated genes related with sugars and sterol metabolism: aldehyde dehydrogenase 1 family, member B1 (g), dolichyl-phosphate β-glucosyltransferase (h) and sorbitol dehydrogenase (i). Nfkb pathway-related gene tumor necrosis factor (ligand) superfamily, member 9 was upregulated (j). PMS1 homolog 1, mismatch repair system component gene, was downregulated (k). And Jumonji domain-containing 6 gene was upregulated (l). m–o Relative gene expression of pluripotency genes (OCT4, NANOG and SOX2). Three qRT-PCR analyses were conducted with each of three independent biological replicates and the data were analysed using a two-sample Student’s t test. The asterisks denote significant statistical differences from untreated control: ^* p ≤0.05; ^** p ≤0.01; ^*** p ≤0.001