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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Global gene expression profiling and senescence biomarker analysis of hESC exposed to H2O2 induced non-cytotoxic oxidative stress

Fig. 6

Abnormal centrosome numbers in H2O2 Hs27 and HUVEC. Hs27 and HUVEC were treated by increasing H2O2 concentrations during 2 h, 24 h and 4 days. Between 250 and 270 cells were counted for each control and/or treatment condition. Both cell types were stained for γ-tubulin (red) and Hoechst 33342 (blue). Where the centrosomes are not visible enough, the area enlarged is shown by a smaller box. In control condition most Hs27 cells display normal number of centrosomes with either one or two centrosomes (c1 and c2). At low H2O2 concentrations cells start to display more than two centrosomal structures, but most still have one or two centrosomal structures (c3, 4 μM). At higher H2O2 concentrations more fibroblasts start to display more than two centrosomal structures (c4, 16 μM). The percentage of Hs27 cells with more than two centrosomes increases sharply between 16 and 64 μM only for 24 h treatment (a). Of the cells displaying more than two centrosomes at 24 h H2O2 treatment, the majority has three centrosomes, and some have four or more (b). Quantification of abnormal centrosomes in HUVEC at different H2O2 timed treatments: in control condition some cells display the correct number of centrosomes (f1, one or two), but the percentage of control cells with more than two centrosomes is higher than in the other analysed cell lines (f2). By the increase of H2O2 even more cells start to display more than two centrosomal structures (f3, 4 μM; f4, 16 μM). The percentage of HUVEC with more than two centrosomes shows no significant differences at any timed treatment between control and H2O2-treated cells (d). In the treated and non-treated cells with more than two centrosomes, the number of centrosomes fragments was from three to five or more (e). Two groups χ2 square test, p ≤0.05

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