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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Cell sex affects extracellular matrix protein expression and proliferation of smooth muscle progenitor cells derived from human pluripotent stem cells

Fig. 3

Expression of estrogen receptors in male and female smooth muscle progenitor cells (pSMCs). a Immunofluorescence staining showed that iPSC-derived pSMCs stained positively for ER-α and ER-β. b Western blot analysis for ER-α and ER-β protein in male HuF1-pSMCs and female HuF5-pSMCs. HuF1-pSMCs and HuF5-pSMCs expressed equivalent amounts of ER-α, while the expression of ER-β in female iPSC-pSMCs showed greater than twofold increase relative to that in male iPSC-pSMCs. Representative blots from three separate experiments performed in triplicate. # p < 0.05 by Student’s t test. Data shown represent the mean ± SD from three independent experiments, each performed in triplicate. ER estrogen receptor, DAPI 4,6-diamidino-2-phenylindole, F female, M male

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