Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Cell sex affects extracellular matrix protein expression and proliferation of smooth muscle progenitor cells derived from human pluripotent stem cells

Fig. 6

Effect of cell sex on the expression of ECM proteins. a, b Expression levels of collagen IαI and collagen IIIαI genes in two pairs of pSMCs were examined by qRT-PCR. At baseline (no E2), female pSMCs had a significantly higher expression of collagen IαI gene, compared to the corresponding male pSMCs. E2 treatment induced collagen IαI and collagen IIIαI gene expression in female pSMCs in a dose-dependent manner, but not in male pSMCs. c, d Western blot analysis of collagen III protein in male and female pSMCs. E2 treatment induced collage IIIαI expression in female pSMCs, but not in male pSMCs. *p < 0.05, vs untreated cells; data analyzed by one-way and two-way ANOVA followed by Tukey post-hoc test. # p < 0.05, male pSMCs vs female pSMCs by Student’s t test. Data shown represent the mean ± SD from three independent experiments, each performed in duplicate (a, b) or triplicate (c, d). pSMC smooth muscle progenitor cell, F female, M male, MMP matrix metalloproteinase, TIMP tissue inhibitor of metalloproteinase

Back to article page