Fig. 3
From: Influences of age-related changes in mesenchymal stem cells on macrophages during in-vitro culture
Cell morphology (a) and immunofluorescence (b) of RAW264.7 cells following coculture with YMSCs and AMSCs (with or without IFNγ stimulation); RAW264.7 cells without coculture served as control. a Images of RAW264.7 cells in different groups were obtained with an inverted microscope (scale bar: 100 μm). b Specific markers for the proinflammatory phenotype (iNOS) and anti-inflammatory phenotype (CD206) were stained via immunofluorescence (iNOS and CD206 were labeled with Alexa Fluor® 594 secondary antibodies, cytoskeleton was labeled with FITC-phalloidin, cell nucleus was labeled with Hoechst; scale bar: 100 μm). AMSC aged bone marrow-derived mesenchymal stem cell, YMSC young bone marrow-derived mesenchymal stem cell, iNOS inducible nitric oxide synthase