Fig. 6
From: Influences of age-related changes in mesenchymal stem cells on macrophages during in-vitro culture
Phagocytosis of CRBCs by RAW264.7 cells following coculture with YMSCs and AMSCs (with or without IFNγ stimulation); RAW264.7 cells without coculture served as control. a Romanowsky staining for RAW264.7 cells after being incubated with CRBCs (red arrows, CRBCs being phagocytosed by RAW264.7 cells). b Fluorescence staining of RAW264.7 cells after being incubated with CRBCs; CRBCs were labeled with PKH-26 before being phagocytosed, and RAW264.7 cells were labeled by Hoechst following phagocytosis (scale bar: 100 μm). c Flow cytometric analysis of RAW264.7 cells (stained by FITC-F4/80 following phagocytosis) and CRBCs (labeled by PKH-26 before incubation). Data shown as mean ± standard deviation. NS p > 0.05, no significant difference between the indicated groups. AMSC aged bone marrow-derived mesenchymal stem cell, YMSC young bone marrow-derived mesenchymal stem cell (Color figure online)