Fig. 7

Cell migration of RAW264.7 cells in Transwell systems during (a) or following (b) coculture with YMSCs and AMSCs (with or without IFNγ stimulation); RAW264.7 cells without coculture served as control. a BMSC chemotaxis migration assay: RAW264.7 cells were placed in the inserts (upper compartments), while BMSCs were placed in the plates (lower compartments); representative images (scale bar: 100 μm) were taken 10 h after transporting RAW264.7 cells to the Transwell systems (BMSCs were preattached in the lower plate), and statistical analysis was performed by counting the number of migrated cells in different groups. b Mφ migration assay: RAW264.7 cells following coculture (without coculture as control) were placed in the inserts (upper compartments), while no cells were placed in the plates (lower compartments); representative images (scale bar: 100 μm) were taken 10 h after transporting RAW264.7 cells to the Transwell system, and statistical analysis was performed by counting the number of migrated cells in different groups. *p < 0.05, ***p < 0.001, a significant difference in the number of migrated cells compared to control; ^△△△ p < 0.001, a significant difference between the indicated groups; ^NS p > 0.05, no significant difference between the indicated groups. AMSC aged bone marrow-derived mesenchymal stem cell, YMSC young bone marrow-derived mesenchymal stem cell