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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Umbilical cord-derived mesenchymal stromal cells: predictive obstetric factors for cell proliferation and chondrogenic differentiation

Fig. 1

Characterization of WJ-MSC. a Viability, apoptosis, and necrosis were evaluated just after thawing. Apoptosis and necrosis of cells was analyzed by flow cytometry using the Vybrant/Apoptosis™ kit based on the AnnexinV/ PI staining procedure. b Phenotypic analysis, c clonogenicity assays (scale bar = 1 cm), and d multilineage differentiation (scale bar = 100 μm) were performed at the end of P2. For mesenchymal surface markers, the results are shown as percentages of positive cells. Osteogenic differentiation was evaluated by the matrix calcium mineralization as shown by Alizarin red staining. Adipogenic differentiation was assessed by detection of lipid droplets by a fluorescent staining with AdipoRed™ (n = 5)

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