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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds

Fig. 3

Equine MSC secrete stable, low molecular weight factors that inhibit bacterial growth. a Relative growth of E. coli (left panel) and S. aureus (right panel) based on absorbance readings at 600 nm. Cultures were grown in DMEM, MSC CM, heat-inactivated MSC CM (HI) or proteinase K-treated CM (PK) each diluted 1:2 in LB broth. b Relative growth of E. coli (left panel) and S. aureus (right panel) based on absorbance readings at 600 nm. Cultures were grown in DMEM, MSC CM, frozen-thawed MSC CM (FT) or lyophilized-reconstituted CM (Lyoph) each diluted 1:2 in LB broth. c Relative growth of E. coli (left panel) and S. aureus (right panel) based on absorbance readings at 600 nm. Cultures were grown in DMEM, MSC CM, and MSC CM fractioned by size of secreted factors. Different letters indicate statistically significant (P < 0.05) differences. n = 3. Abx antibiotics, CM conditioned medium, DMEM Dulbecco’s modified Eagle medium, MSC mesenchymal stromal cells

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