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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds

Fig. 5

Equine MSC express at least four AMPs. a Detection of three AMPs in MSC CM and control CM as detected by a commercially available antibody array. n = 1. b (i) Relative band densities of five AMP transcripts in equine MSC and NBL6 compared to the reference transcript B2M as detected by RT-PCR. (ii) Representative images of PCR products run on agarose gels. Lane 1 = molecular weight marker; Lanes 2, 3, and 4 = equine MSC derived from three different horses; Lane 4 = NBL6. c (i) Relative band densities of four AMPs in equine MSC and NBL6 compared to the reference protein β-actin as detected by Western blot. (ii) Representative images of protein lysates run on polyacrylamide gels, transferred to PVDF membranes, and probed with anti-AMP antibodies. Lane 1 = molecular weight marker; Lanes 2, 3 and 4 = equine MSC derived from three different horses; Lane 4 = NBL6. d Images of equine MSC labeled with four anti-AMP antibodies (red). Scale bars = 10 μm, *P < 0.05, n = 3. CM conditioned medium, MSC mesenchymal stromal cells

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