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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds

Fig. 6

Blocking AMP activity decreases the effects of MSC CM on bacteria. a Relative growth of E. coli (left panel) and S. aureus (right panel) based on absorbance readings at 600 nm. Cultures were grown in DMEM, MSC CM, and MSC CM pre-incubated with anti-AMP antibodies (Ab) or MSC CM pre-incubated with an isotype control antibody, each diluted 1:2 in LB broth. b Incorporation of NPN into depolarized E. coli and S. aureus membranes in the presence of decreasing concentrations of DMEM, MSC CM, and MSC CM pre-incubated with anti-AMP antibodies or MSC CM pre-incubated with an isotype control antibody as measured by fluorescent emission at 444 nm (i and iii). Emission values from E. coli and S. aureus with treatments diluted 1:2 (ii and iv). Different letters indicate statistically significant (P < 0.05) differences. n = 3. CFU colony-forming units, CM conditioned medium, DMEM Dulbecco’s modified Eagle medium, MSC mesenchymal stromal cells

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